抄録
These few years, many investigators have been studying hemofiltration (HF) and hemodiafiltration (HDF) as one of methods for treating chronic uremia. It is said that HF and HDF have high efficiency in eliminating middle molecular substances from blood. However, the appropriate method for evaluating the characteristic curve in cut-off molecular weight of a membrane and the definite value of the cut-off MW have not been presented.
We measured the cut-off MW in a different kind of HF and HDF membranes with using blood and normal saline solution, and obtained the following favorable results.
1) When the cut-off MW of HF and HDF membranes was measured in blood and normal saline solution, the cut-off MW in blood was lower than that in the normal saline solution for each of the membranes.
2) It seems that the major factors in decreasing the cut-off MW in blood are concentration polarization and the clogging on the surface of membrane, and not the protein-binding of the indicated substances.
3) The cut-off MW was not affected by a change in share rate, transmembrane pressure and circulation time.
4) The adsorption of the indicated substances on the membrane was observed in some kind of membranes. Therefore, it is absolutely important to collect the samples after circulating blood effectively and sufficiently in order to measure the cut-off MW value accurately.
5) Our method using blood is applicable to the evaluation of HF and HDF membrane, and our gel chromatography method may be recommended as an effective method for measuring the indicated substances in plasma and blood.
