Abstract
To develop a simple pretreatment and reversed-phase chromatography method for β-carotene in edible seaweeds, optimum HPLC column and high efficient extraction method were selected. Beta-carotene measurement was performed at 450 nm. A TSKgel ODS-80TS column seems to be a suitable column for β-carotene analysis among three columns tested. Suitable extraction solvent for high efficiency extraction was ethanol containing 10% pyrogallol among extraction solvents tested. The present method was successfully applied to β-carotene analysis and should prove useful for β-carotene analysis in edible seaweeds.
