Abstract
A physiological or an ecological investigation sometimes suggests
potentially cryptic species. Taxonomic study such as morphological and
molecular analysis are required to verify the independency of the cryptic
species. Construction of phylogenetic tree is an essential part of the
molecular analysis. However, the opportunity is limited for applied
phycologists to learn the principal and procedure of the phylogenetic
analysis. Therefore, here we summarized the basic knowledge of
phylogenetic analyses including 1) construction of molecular dataset, 2)
characters of the currently employed methods, and 3) selection of
appropriate method. In the final section, we described the procedure to
perform maximum likelihood tree using a software MEGA.
