Abstract
Since the development of next-generation sequencing (NGS) technology, tremendous progress has been made in its sequencing capabilities and cost reduction. The application of these technologies to the study of macroalgae will greatly help our understanding of their taxonomy and ecology, as well as their application to aquaculture. We conducted RNA-seq analyses of the reference strain U51 and the high water temperature tolerant strain MET11 to research genes that responsive to high temperature stress in the marine red algae Neopyropia yezoensis (susabi-nori). The results showed clear differences in the expression levels of genes involved in high temperature stress between U51 and MET11. On the other hand, we also investigated whether there are genetic differences between male and female cells on the same blade by RNA-seq and single nucleotide polymorphism (SNP) analysis using the marine red alga Pyropia katadae. However, these analyses have yet to find genetic differences between male and female cells, and we would like to try other approaches such as genomic analysis. Although our research has just begun, we hope to contribute to the study of macroalgae through NGS-based analysis in the future.
https://ror.org/01529vy56 
