Journal of Advanced Science
Online ISSN : 1881-3917
Print ISSN : 0915-5651
ISSN-L : 0915-5651
Letter
“Sniffer-camera” using enzyme reaction for visualization of transpired ethanol from palm skin
Kenta IITANIToshiyuki SATOXin WANGKoji TOMATakahiro ARAKAWAKohji MITSUBAYASHI
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2014 Volume 26 Issue 3+4 Pages 20-22

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Abstract

An imaging system for gaseous ethanol transpired from human palm skin, based on an enzymatic reaction, was assembled and validated. This system uses a highly sensitive camera that measures gaseous ethanol concentrations as intensities of chemiluminescence from luminol’s reaction induced by alcohol oxidase (AOD) and a luminol-hydrogen peroxide-horseradish peroxidase (HRP) system. Conversion of gaseous ethanol concentrations and distributions proceed on an enzyme-immobilized mesh substrate with luminol solution in a dark box. In order to visualize ethanol transpired from human palm skin, we improved the chemiluminescence sensitivity of the imaging system with a mixture of a high-purity luminol solution of luminol sodium salt HG solution and an enhancer of eosin Y solution. The detection limit of the ethanol concentration was 4.9 ppm. This highly sensitive imaging allows successful visualization of ethanol transpired from palm skin.

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