Abstract
The effects of different cytokinin levels on multiplication of axillary shoots during culture on agar medium and subsequent growth of shoots in liquid shake culture were examined. The number of axillary shoot buds was highest(about 100) in culture medium containing 1mg/l of 4PU (one kind of cytokinin).
Tissue segments with multiple shoot buds thus obtained were further grown in liquid medium by shake culture. The growth of shoot buds into plantlets in shake culture was examined for liquid media containing 0, 0.1 and 1mg/l 4PU in combination with 0, 0.1, 0.3 and 1mg/l ABA. The growth was most faborable in the medium containing 1.0mg/l 4PU in combination with 0.1mg/l ABA. Individual shoots grown under this condition were easily separated by hands, transplanted, and established satisfactorily in soil.
