腸炎菌加熱死菌免疫マウス細胞の移入による免疫の研究

I. 細胞移入により附与される正常マウス腹腔内菌清掃現象

抄録

We have elucidated in a previous study that, in mice actively immunized with killed vaccine or passively immunized with anti-O serum, intraperitoneally challenged virulent organisms of S. enteritidis were cleared from their abdominal cavities during an early stage of the infection, although these immunizing. procedures did not save mice from death after the infection.
In the present study it was clarified that the clearance of infecting bacteria in the peritoneal cavity was also achieved by transfer of cells from killed vaccine-immunized mice. The tentative conclusion we have reached, is that the phenomenon is mediated by the participation of transferred antibody producing, cells, and the synthesis of O-antibodies in the recipients is an essential feature of this phenomenon. This conclusion was supported by the following facts.
1. Peritoneal exudate cells, especially rich in macrophages, obtained from killed vaccine-immunized. mice conferred the most potent “clearing capacity” on recipient mice.
Cells from the spleen or lymph nodes of immunized mice also could transfer the clearing capacity, but those from the liver could not.
2. Intravenous, intraperitoneal and subcutaneous routes were all effective in transferring the capacity.
3. The capacity was transferred only by live cells, being inactivated by treatments of cells with. freezing-thawing, mild heating, and sonic oscillation.
4. The clearance was effective only when the antigen used for immunizing donors and challenging bacteria had common specific O-antigen.
5. Circulating antibody of more than 1: 20 agglutinin titer appeared in the serum of recipients 1 to. 4 days after transfer, when the cells collected from donors were secondarily stimulated in vitro by incubation with the heat killed Salmonella before transfer.
6. The clearing capacity transferred by cells decreased in its intensity within a week or so after transfer, when the transfer was made into homologous adult mice.