Abstract
I have investigated the growth supporting abilities of three synthetic media for the growth of S. pullorum and S. gallinarum Used strains were 35 strains of S. pullorum and 11 of S. gallinarum Tested synthetic media were those reported by Yasushi (1959), Schoenhard and Stafseth (1953) and it's modification by Gilfillan et al.(1955).
Many of the used strains of S. pullorum could grow moderately in the composition of Yasushi, but in the other two media only six strains showed good growth, the growth of the others were scanty or none.
The reason of this difference of growth response was explained by the fact' that many strains of S. pullorum required proline and glutamic acid for their growth, and the two mediaother than Yasushi's lack these amino acids.
All tested strains of S. gallinarum except one showed good growth in three media. Therefore, the growth requirement of S. gallinarum seems more non-exacting than S. pullorum.
5 strains of S. pullorum could not grow fairly in the Yasushi's composition, and a modification of Yasushi's medium was attempted. As the results, it was confirmed that the addition of histidine and methionine, increase of the added amount of glucose were effective for the increase of growth.
One strain of S. pullorum, strain No.21, could not grow even in the modified composition of Yasushi's medium, the growth requirement of which must be investigated in future.
