異常長大菌の生理学的研究
III. ペニシリン長大菌の核酸および紫外線不活化について
1965 年 20 巻 2-3 号 p. 70-75
詳細
1965 年 20 巻 2-3 号 p. 70-75
Salmonella typhi H 901 w cells developed in abnormally elongated filament cells in presence of penicillin. The ultraviolet inactivation kinetics of the filament cells were examined in this paper to investigate the relationship with nucleic acids component and chromatinic bodies in these organisms.
1. Throughout the process of the filament formation, DNA and RNA synthesis proceeded without inhibition.
2. The composition of DNA of normally harvested Sal. typhi H 901 w was analysed by a method of phenol extraction in 2M NaCl soltuion. The result was that, Adenine; 22.75, Guanine; 27.15, Cytosine; 27.75, Thymine; 22.35. In contrast with this finding, concerning the result of the base analysis in the filament cells, no reasonable accordance was attained in four trials performed separately.
3. By a method of ultraviolet inactivation, no remarkable increase of target number in the filament cells was observed in no proportion to multiplement of chromatinic bodies in the cells.
4. Seventy per cent of biomass of the filament cells revived after transfered to a fresh medium without penicillin.
The finding of this study suggest a probability that some extra-nuclear factor would take a part in the mechanism of cell division.
