Studies on the Prophylaxis Against Habu (Trimeresurus flavoviridis) Bite by Snake Venom Toxoid

2. Studies on the Side Reaction of Toxided Venom by the Plantar Subcutaneous Inoculation of Mice

Abstract

Habu venom treated with the same amount of dihydrothioctic acid (DHTA) at 37°C for 30 minutes lost its hemorrhagic and necrotic action but remained some swelling of foot of a mouse which was inoculated with the toxoided venom subcutaneously into the plantar side of foot. The degree of swelling due to the venom which was harvested from Okinawa was more marked than that of venom from Amami Islands. A precipitation occurred after the mixture of Amami venom with DHTA was more dense than that of venom from Okinawa. The swelling decreased after the freeze-drying of the toxoided venom without loss of its antigenicity.