日本細菌学雑誌
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
芽胞形成菌におけるDipicolinic Acid生合成および蓄積機構に関する研究
I. Diketopimelate-NH3 ComplexのDipicolinic Acid生成反応に関する活性物質の物理的,化学的性状
川崎 近太郎桜井 純近藤 雅臣
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1968 年 23 巻 11 号 p. 772-776

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An active fraction catalyzing synthesis of dipicolinic acid from the diketopimelate-NH3 complex was extracted from sporangium. Some physical and chemical properties of the fraction were investigated. The activity of this fraction was stable to heat treatment at 100°C for 2 hours and also to the proteinase digestion. The active fraction was dialysable against the cellophane and collodion membrane. When the effects of various reducing agents on the activity of the fraction were compared, it was found that the addition of β-mercaptoethanol to the reaction system showed a significant increase of dipicolinic acid formation. PCMB and monoiodeacetate partially inhibited the reaction. KCN completely inactivated the fraction. Neither acid hydrolysate nor ash of the fraction showed any activity.
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