抄録
Studies were made to clarify the immunochemical properties and sugar constituents of K antigens of Vibro parahaiemolyticus. Two strains belonging to the O group 2, the Nakatsukawa (O 2:K 3) and the T-1698 (O 2:K 28), were employed. Purification was made by means of phenol-water extraction, several times of freezing and thawing to remove nucleic acid and ultracentrifugation.
The properties of the K substances finally obtained were as follows.
1. K substances showed high degrees of serological specificity when they were subjected to reaction with homologous anti-K sera by the Ouchterlony gel-diffusion method.
2. Contamination with O antigen or any other antigenic substance in these final products were denied by the Ouchterlony method, although the K substances gave two specific precipitation lines.
3. These two K substances were considered to be a kind of hapten. When rabbits were injectedwith 80mg of the substance, no detectable K antibody was produced in them. Besides, the substances showed no toxic effect on mice.
4. Chemical analyses of these substances revealed that each substance was an acidic polysaccharide which consisted mainly of galactose and rhamnose, and that it contained no trace of such acidic sugars as uronic acid and sialic acid, in spite of the acidic characteristics it exhibited.
