Abstract
Normal growth, formation of filament forms, and recultivation of the filament forms were studied on Pseudomonas aeruginosa with the aid of the motion picture. The results obtained are as follows.
1) P. aeruginosa changed into filament forms and spheroplasts when cultivated on an agar medium containing 100mcg/ml of sulfobenzyl penicillin (SB-71).
The spheroplasts had balloon-like projections, which increased gradually in size with cytoplasm moving into them. Finally, the projections bursted.
2) Filament forms collected from the medium containing SB-71 and cleaned by washing with M/20 phosphate buffer (pH 7.2) were recultivated on the renewed agar medium. Normal forms of P. aeruginosa appeared after one another by division from both sides of each filament form.
