Abstract
Acriflavine-treated substrains of the Clostridium perfringens type D strain Lam 61 were examined for colonial morphology, biochemical properties, toxigenicity, sporulation, and antigenic properties. On the basis of the difference in the colonial morphology on Zeissler's plate, 10 substrains were established. Of these mutants, seven were ε-toxigenic and the remaining three non-toxigenic. Further studies revealed that the rougher the colonies of these mutants, the great the length of the cells, and that some of these mutants were deficient in ability to ferment several sugars, had an attenuated ability to sporulate, and were more agglutinable spontaneously than the parent strain.
