Abstract
Lipopolysaccharide (LPS) antigens were purified from heated cell extracts of Pseudomonas aeruginosa types A, B, D, E, and I. Indirect hemagglutination (IHA) with a given type of them showed specific reactivity to anti-Pseudomonas rabbit serum immunized with the same type of antigen.
As a result of absorption of heterotypic antibody, type-specific antibody in human serum was titrated successfully by using LPS-coated sheep erythrocytes. Sera from healthy adults and patients infected with P. aeruginosa were studied subsequently and revealed to possess antibodies against P. aeruginosa.
IHA antibody against type E wasresistant to 2-mercaptoethanol (2-ME) and classified as IgG in patients infectedwith type E of Pseudomonas. It was however, sensitive to 2-ME and classified as IgM in patients infected with any other type of P. aeruginosa, as well asin healthy adults. The titer and positive incidence ofantibody against type-E Pseudomonas were higher than those of antibody against any other type of Pseudomonas examined.
