Abstract
Sporulating cells of Bacillus subtilis 60015 exhibited calcium uptake. Ca uptake by them was stimulated by dicyclohexylcarbodiimide (DCCD). The stimulating effect of DCCD appeared in the stationary stage and increased with the lapse of time until 9hr of incubation, when it began to decrease. In the sporulating cells Ca uptake at 0C was about half of that at 37C. On the contrary, vegetative cells took up Ca at 0C, but did not take it up at 37C. Ca uptake of the sporulating cells was inhibited completely by carbonylcyanide m-chlorophenylhydrazone (CCCP), KCN or NaN3, and to about 50% by these inhibitors in the presence of DCCD.
On the basis of these results, the mechanism of Ca uptake by the sporulating cells was discussed.
