Monoclonal Antibody Against Human HLA Class II Antigens

II Analysis of immunoglobulin-production system using a monoclonal antibody

Abstract

To investigate the role of HLA class II antigens in the immunoglobulin-production system, the monoclonal antibody Sa3 against HLA class II antigens was added to the immunoglobulin-production system and detection of immunoglobulin secreting cells was performed by plaque assay with Protein A-coated sheep red blood cells. The results are as follows;
1. Sa3 manifested profound inhibitory effect on Pokeweed mitogen-(PWM) induced immunoglobulin synthesis.
2. The B cells treated with Sa3 in co-culture T cells did not produce immunoglobulin on PWM-induced immunoglobulin synthesis.
3. Sa3 did not give any influence on induction of immunoglobulin secretion in B cell-differentiation factor (BCDF)-responsive CESS cells.
Thus, HLA class II antigens were proven to play an important role in immunoglobulin-production system.