Monoclonal antibody mNI-11 strongly induces homotypic cell aggregation of monocyte-like cell line U937
effects of conditioned medium and cytochalasin B
1994 Volume 49 Issue 5-6 Pages 787-791
Details
1994 Volume 49 Issue 5-6 Pages 787-791
A mouse monoclonal antibody (mAb), designated as mNI-11, has been produced. This mAb strongly induced homotypic cell aggregation (HCA) of monocyte-like cell line U937 cultured with conditioned medium derived from PHA-stimulated peripheral blood mononuclear cells and cytochalasin B. HCA occurred at an optimal temperature of 37C but not 4C. mAbs to CD 18 (lymphocyte function-associated antigen-1β; LAF-1β) and CD 54 (intercellular adhesion molecule-1; ICAM-1) completely inhibited this HCA. These results suggest that mNI-11 strongly induces HCA of the U937 cells in an activated form after stimulation with conditioned medium and cytochalasin B.
