2010 Volume 12 Issue 3 Pages 73-80
A Chinese chive (Allium ramosum L.) cultivar, ‘Tender Pole’, which shows parthenogenesis (P), was crossed with a non-parthenogenic strain ‘97-12-102’. The resultant F1 population was used to develop Parthenogenesis-Linked Markers (PLM) by the bulk segregant method. Previous data showed that the phenotype of parthenogenesis was dominant. Finally, 1,443 random primers were studied to screen a pair of bulked DNA, which were derived from the parthenogenesis group and non-parthenogenesis group of F1 plants. Three RAPDs were selected as parthenogenesis bulk-specific markers. Then, these were cloned using the TA cloning technique and sequenced to convert the clones to SCAR markers, named PLM1, PLM2 and PLM3. Segregation of these markers was examined in the F1 population. It was confirmed that two of these markers PLM1 and PLM3 were linked to parthenogenesis at distances of 3.4 cM and 1.1 cM respectively. In contrast, PLM2 was not linked to parthenogenesis, but seemed to be concerned with a high degree of parthenogenesis. PLM1 and PLM3 were widely conserved in 73 Chinese chive variety collections. However, ‘Seito’ and its progeny ‘00-02-05’, which show parthenogenesis, did not demonstrate either of these markers.
