Abstract
Three polymorphic loci selected from 5 microsatellite loci isolated from E. colona and 10 microsatellite loci isolated from the chloroplast and nuclear genome of rice were studied by analyzing 170 samples of Japanese Echinochloa spp. Three microsatellite loci revealed a total number of 16 alleles and the DNA sequence of each allele was determined. One hundred and sixty-nine samples of Japanese Echinochloa spp. were classified into 14 types by the combination of these alleles. The samples of the cultivated species E. esculenta were classified into only 2 types among 11 types of the ancestral species E. crus-galli. A tetraploid species E. oryzicola was distinguished from hexaploid species. Moreover, the polymorphisms of ISSR classified 143 Japanese Echinochloa spp. samples into 14 types. E. esculenta showed only one type among 9 types of E. crus-galli. These results suggest that E. esculenta was domesticated from a limited part of the E. crus-galli population.
