Construction of a High-density AFLP and SSR Map Using Recombinant Inbred Lines of Cultivated × Weedy Soybean

Abstract

A genetic linkage map of soybean was constructed by using a cross between a cultivar ‘Keburi’ and a weedy form of soybean ‘Masshokutou Kou 502’. The parents differed in their ability in somatic embryogenesis. The mapping population consisted of 117 recombinant inbred lines (F₁₁), and generated 30 linkage groups, which covered 2089 cM, including 515 amplified-fragment-length polymorphism (AFLP) markers and 85 simple sequence repeat (SSR) markers. For AFLP analysis, a simple technique using small polyacrylamide gels combined with silver staining was applied, which enabled a single person to construct a map in a short time at a reasonable cost. Comparison of this map with previously published maps revealed that 1) the level of polymorphism was the same as in a cultivar × cultivar cross; and 2) the cultivar × weed map was 13 % shorter than the cultivar × wild map. The high-density map constructed in this study would contribute to the molecular mapping of quantitatively and qualitatively inherited characters from weedy forms of soybean.