Abstract
Recent advances in DNA marker technologies have increased the use of single nucleotide polymorphism (SNP) as a genetic marker. We developed SNPs in Paraserianthes falcataria and arranged them for multiplexed DNA typing using single nucleotide primer extension (SNuPE). Seventeen sequence characterized amplified regions (SCARs) were developed from random amplified polymorphic DNA (RAPD). In 12 SCARs of them, a SNP which possessed high heterozygosity was selected, and three sets of multiplexed SNuPE analysis system with 4 SNPs were constructed. Estimating the discrimination power (DP) revealed that set A had the highest DP (0.968), followed by set B and set C. The ability to discriminate could be almost 100% (1.000 of DP) when all sets were used. This SNuPE system provides a practicable method for individual identification of this forest tree species.
