Breeding Science
Online ISSN : 1347-3735
Print ISSN : 1344-7610
ISSN-L : 1344-7610
Note
Natural diversity of seed α-tocopherol ratio in wild soybean (Glycine soja) germplasm collection
Maria Stefanie DwiyantiShouhei MaruyamaMari HironoMasako SatoEuiho ParkSei Hyung YoonTetsuya YamadaJun Abe
Author information
JOURNALS OPEN ACCESS FULL-TEXT HTML
Supplementary material

2016 Volume 66 Issue 4 Pages 653-657

Details
Abstract

Alpha-tocopherol is one of four tocopherol isoforms and has the highest vitamin E activity in humans. Most cultivated soybean seeds contain γ-tocopherol as the predominant form, and the ratio of α-tocopherol content to total tocopherol content (α-tocopherol ratio) is <10%. Three soybean accessions from Eastern Europe have α-tocopherol ratios of >20%. This higher content is likely due to mutations in the promoter region of the γ-tocopherol methytransferase-3 (γ-TMT3) gene. We surveyed a wild soybean germplasm collection and detected 16 accessions with stable seed α-tocopherol ratios of >20% under different growth conditions. The α-tocopherol ratios were greatly reduced when the plants were grown under cool temperatures during seed maturation, but increased to varying degrees at higher temperatures. Sequence analysis of the γ-TMT3 promoter of 11 of the accessions identified four haplotypes, one of which corresponded to that of cultivars with higher contents. These wild accessions can thus serve as novel donors for breeding cultivars with high α-tocopherol ratios and for better understanding the genetic basis of α-tocopherol synthesis in soybean.

Information related to the author
© 2016 by JAPANESE SOCIETY OF BREEDING
Previous article
feedback
Top