2022 年 72 巻 3 号 p. 274
Breeding Science 70: 462–473 (2020)
In the above article, there was a mistake in Table 3.
The sequence of reverse primers was presented in the 3′→5′ direction (highlighted).
| Marker name | SNP position (bp) | Marker type | Primer sequence (5ʹ→3ʹ) | Tm (°C) | Restriction enzyme |
Expected size (bp) | ||
|---|---|---|---|---|---|---|---|---|
| Susceptible | Resistant | |||||||
| RsR6-1 | 24,669,159 | CAPS | F: | GGAAATATTGGTTACAATCCAGTG | 57.5 | MnlI | 227 | 173, 54 |
| R: | GAATACAACAAATCACTACCGGTC | 59.3 | ||||||
| RsR6-2 | 34,389,374 | CAPS | F: | CTTCTTGATAGGACGACGTGATAT | 59.3 | RsaI | 87, 116 | 203 |
| R: | CAATCAACGGATCACCCATTTTTC | 59.3 | ||||||
| RsR6-3 | 34,399,541 | CAPS | F: | CTCTTTTTGCCAGATCTTGAATAG | 57.5 | MnlI | 214 | 116, 98 |
| R: | CCATAGGTCAGCATCAAATTTCAA | 57.5 | ||||||
| RsR6-4 | 35,950,028 | CAPS | F: | GTTTTCCTTGCAAATCATTTTGGC | 57.5 | MseI | 116, 97 | 213 |
| R: | GTATATGTTGAGTTCACAATTCCC | 57.5 | ||||||
| RsR6-5 | 37,049,726 | CAPS | F: | CTCAGAAACTGGATAAACTCGAAG | 59.3 | HinfI | 204 | 129, 75 |
| R: | GGAGAAAGCAGCCAGCCATTTTT | 60.6 | ||||||
| RsR6-6 | 37,186,202 | dCAPS | F: | CGGTGATGAGCAGGATTGATAAAA | 59.3 | HpyCH4III | 234 | 200, 34 |
| R: | AGTCTTGGCCTTTGACGTGAAAGTGACACAAGAAG | 60.6 | ||||||
| RsR12-1 | 2,941,301 | dCAPS | F: | GTTACACGAACAAGCTTAAATTTCTAGATTTATCCC | 58.8 | AciI | 203 | 168, 35 |
| R | GTAATCAATTCGAAGGACCTGTC | 64.9 | ||||||
Now the sequences are reverse complemented and corrected in the 5′→3′ direction (highlighted).
| Marker name | SNP position (bp) | Marker type | Primer sequence (5ʹ→3ʹ) | Tm (°C) | Restriction enzyme |
Expected size (bp) | ||
|---|---|---|---|---|---|---|---|---|
| Susceptible | Resistant | |||||||
| RsR6-1 | 24,669,159 | CAPS | F: | GGAAATATTGGTTACAATCCAGTG | 57.5 | MnlI | 227 | 173, 54 |
| R: | GACCGGTAGTGATTTGTTGTATTC | 59.3 | ||||||
| RsR6-2 | 34,389,374 | CAPS | F: | CTTCTTGATAGGACGACGTGATAT | 59.3 | RsaI | 87, 116 | 203 |
| R: | GAAAAATGGGTGATCCGTTGATTG | 59.3 | ||||||
| RsR6-3 | 34,399,541 | CAPS | F: | CTCTTTTTGCCAGATCTTGAATAG | 57.5 | MnlI | 214 | 116, 98 |
| R: | TTGAAATTTGATGCTGACCTATGG | 57.5 | ||||||
| RsR6-4 | 35,950,028 | CAPS | F: | GTTTTCCTTGCAAATCATTTTGGC | 57.5 | MseI | 116, 97 | 213 |
| R: | GGGAATTGTGAACTCAACATATAC | 57.5 | ||||||
| RsR6-5 | 37,049,726 | CAPS | F: | CTCAGAAACTGGATAAACTCGAAG | 59.3 | HinfI | 204 | 129, 75 |
| R: | AAAAATGGCTGGCTGCTTTCTCC | 60.6 | ||||||
| RsR6-6 | 37,186,202 | dCAPS | F: | CGGTGATGAGCAGGATTGATAAAA | 59.3 | HpyCH4III | 234 | 200, 34 |
| R: | CTTCTTGTGTCACTTTCACGTCAAAGGCCAAGACT | 60.6 | ||||||
| RsR12-1 | 2,941,301 | dCAPS | F: | GTTACACGAACAAGCTTAAATTTCTAGATTTATCCC | 58.8 | AciI | 203 | 168, 35 |
| R | GACAGGTCCTTCGAATTGATTAC | 64.9 | ||||||
