Identification of a major QTL conferring resistance to wheat yellow mosaic virus derived from the winter wheat ‘Hokkai 240’ on chromosome 2AS

Abstract

Wheat yellow mosaic disease is a soilborne disease caused by wheat yellow mosaic virus (WYMV). Symptoms include yellow mosaic coloring of leaves, stunting, and growth inhibition. Here we conducted a detailed analysis of resistance to this virus in winter wheat ‘Hokkai 240’ by carrying out inoculation tests of WYMV and conducting field tests. The resistance level observed in ‘Hokkai 240’ was compared with those in varieties harboring known resistance genes. In the inoculation tests, ‘Hokkai 240’ showed resistance to WYMV Pathotypes I and II and partial resistance to Pathotype III. This result was contrary to the sensitive responses to the three pathotypes exhibited by the variety harboring resistance gene on chromosome 2DL. In fields infected with WYMV Pathotypes II and III, ‘Hokkai 240’ plants exhibited few disease symptoms and little proliferation of the virus. By analyzing the quantitative trait loci (QTLs) in recombinant inbred lines from a cross between ‘Hokkai 240’ and ‘Nanbukomugi’, a single major QTL, Q.Ymhk, from ‘Hokkai 240’, which had significant effects on Pathotypes II and III of the virus, was detected in the proximity of snp4212 and snp4215 mapped on chromosome 2AS. These results indicate that Q.Ymhk may be useful for developing broad resistance to WYMV in wheat breeding programs.