Breeding Science
Online ISSN : 1347-3735
Print ISSN : 1344-7610
ISSN-L : 1344-7610
Notes
A new sweetpotato variety ‘Michishizuku’ for alcohol and starch production with high yield and resistance to foot rot
Akira Kobayashi Yukari KawataTakeo SakaigaichiKeisuke SuematsuYumi KaiTetsufumi SakaiYasuhiro Takahata
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2026 年 76 巻 2 号 p. 196-203

詳細
Abstract

We developed a new sweetpotato variety, ‘Michishizuku’, as the raw material for imo-shochu, a traditional Japanese distilled liquor made from sweetpotaoes, and for starch production. Michishizuku was selected from a cross between ‘Konaishin’ and Kyukei 09187-14 and was released in 2021. Its total yield was higher than that of ‘Koganesengan’, slightly higher than that of ‘Shiroyutaka’, and slightly lower than that of Konaishin. The starch content of Michishizuku was significantly higher than those of the other varieties, and its starch yield was 146–147% that of Koganesengan and 93–102% that of Konaishin. Shochu brewing tests confirmed that the alcohol yield of Michishizuku was higher than that of Koganesengan. In addition, the flavor of shochu made from Michishizuku (Michishizuku shochu) was similar to that of Koganesengan shochu, and its sensory evaluation score was slightly higher than that of Koganesengan shochu. Based on these results, Michishizuku is highly suitable for shochu brewing. The viscosity characteristics of the starch of Michishizuku were similar to those of Shiroyutaka and Konaishin, and its whiteness was as high as that of Shiroyutaka. Therefore, Michishizuku is also suitable as a raw material for starch production. Michishizuku exhibited moderately strong resistance to foot rot, which causes severe damage to sweetpotato in the Southern Kyushu area. These characteristics suggest that Michishizuku will contribute to reductions in damage caused by foot rot and the stable production of shochu and starch.

Introduction

Imo-shochu is a Japanese traditional distilled liquor made from sweetpotato (Ipomoea batatas (L.) Lam.) and is popular with consumers in Japan. The shochu industry is thriving in southern Kyushu, and sweetpotatoes as the raw material for shochu production are an important crop in agricultural production and the local economy in this region. The leading variety used for shochu production is ‘Koganesengan’, developed at Kyushu National Agricultural Experiment Station in 1966 (Sakai et al. 1967), and it was the second most cultivated variety in Japan in terms of its cultivated area after ‘Beniharuka’ (Kai et al. 2017) in 2022 (MAFF 2024). Koganesengan exhibits high yield, wide regional adaptability, and is used as a raw material not only for shochu, but also food processing, such as chips, kenpi, and paste. Shochu made from Koganesengan has an excellent flavor of sweetpotato and is superior in terms of the balance between flavor and sweetness. Despite the shortcomings of Koganesengan, such as deep grooves on the storage root caused by a physiological disorder, its poor external shape, and its susceptibilities to a southern root-knot nematode (Meloidogyne incognita), coffee root-lesion nematode (Pratylenchus coffeae), black rot (Ceratocystis fimbriata), soil rot (Streptomyces ipomoeae (Person and Martin) Waksman and Henrici.), and fusarium wilt (Fusarium oxysporum), it is still regarded as the most suitable variety for shochu and has been used for decades as the leading variety.

However, since the first case of foot rot disease in Japan in 2018 (Kobayashi 2019), Koganesengan has suffered extensive damage, such as vine death and storage root deterioration, and the cultivation area of Koganesengan in southern Kyushu has decreased from 7,878 ha in 2018 to 6,585 ha in 2022 (MAFF 2021, 2024), which has severely hindered its stable supply as the raw material for shochu. Foot rot is a soil-borne disease caused by the fungus Diaporthe destruens (Harter) Hirooka, Minosh., and Rossman that is difficult to control. Therefore, the development of a sweetpotato variety that is resistant to foot rot and may be used as a raw material for shochu instead of Koganesengan with the similar characteristic flavor of shochu made from Koganesengan is awaited.

In the present study, we developed a new sweetpotato variety with high yield, resistance to foot rot, and suitability as a raw material for shochu and starch production.

Materials and Methods

Breeding process

The new variety ‘Michishizuku’ was initially termed Kyukei 14195-7, renamed Kyukei 359, and then finally called Kyushu No. 200. This clone was selected from the progenies of a cross between ‘Konaishin’ (Kobayashi et al. 2024), as the female parent, and Kyukei 09187-14, as the male parent. A complete pedigree is shown in Fig. 1. Konaishin is a variety for starch production with high yield and resistance to multiple diseases and pests, including foot rot. Kyukei 09187-14 is a high-yield breeding line with a high dry matter content. When steamed, its storage roots exhibit a powdery texture and good eating quality. In 2015, 370 seeds were sown in a greenhouse, and stem cuttings from each seedling were then transplanted in a field at the Kyushu Okinawa Agricultural Research Center, NARO, Miyakonojo, Miyazaki, Japan (31°45 N, 131°00 E). Based on the size, shape, and number of storage roots, 34 clones were visually selected at harvest. In the following 2 years (2016–2017), the selected clones were propagated from storage roots and further evaluated for agricultural characteristics. A preliminary yield trial was conducted in 2018 using 12.6 m2 plots with two replicates, followed by 3-year yield trials from 2019 to 2021 using 12.6 m2 plots with three replicates. The clonal line selected in 2018 was designated as Kyukei 359 and renamed in 2020 as Kyushu No. 200. Local adaptability was evaluated at the two different locations, Miyazaki and Kagoshima prefectures, in 2019–2020. Performance tests for the recommended varieties were conducted at Miyazaki and Kagoshima prefectures in 2021, and then at 3 farm sites in Miyazaki and Kagoshima prefectures in 2020–2021. The suitability for shochu brewing was evaluated by members of the research group for the processing quality of sweetpotato supported by the Japan Root and Tuber Crops Development Association Inc., Kirishima Shuzo Co., Ltd. (Miyazaki, Japan) for 3 years (2019–2021), and Okuchi Shuzo Co., Ltd. (Kagoshima, Japan) in 2019. Kyushu No. 200 was released as Michishizuku in 2021, and was officially registered by the Ministry of Agriculture, Forestry and Fisheries of Japan in 2024.

Fig. 1.

Pedigree of Michishizuku.

Agronomic characteristics

Morphological traits were evaluated according to the test guidelines of The International Union for the Protection of New Varieties of Plants. The agronomic traits of Michishizuku, Koganesengan, ‘Shiroyutaka’ (Sakamoto et al. 1987), and Konaishin under standard and long-term cultivations were examined in 2019–2022 and 2019–2021, respectively. Shiroyutaka is a main variety for starch production, while Konaishin is a variety for starch production with high yield and resistance to multiple diseases and pests registered in 2022 (Kobayashi et al. 2024). An analysis of variance (ANOVA) of total yield, dry matter content, starch content, starch yield, average size of the storage root, and number of storage roots per hill in standard cultivation was performed. Tukey’s HSD test was used to identify significant differences among varieties at P < 0.05. All analyses were performed in JMP 12 (SAS Institute).

Resistance tests for foot rot disease were conducted in a field infested with foot rot in 2021–2022 according to the method performed by Kobayashi et al. (2025). Resistance tests for a southern root-knot nematode and coffee root-lesion nematode were conducted in fields infested with these nematodes in the Miyakonojo station of KARC/NARO in 2018, 2019, and 2021. The tests for the southern root-knot nematode were carried out in fields where M. incognita, race SP1 was the predominant population, while those for the coffee root-lesion nematode were conducted in fields infested with P. coffeae. Resistance tests for soil rot and black rot disease were conducted by Nagasaki and Tokushima prefectures in 2019, respectively. Resistance test for fusarium wilt (F. oxysporum) was performed using the pot test according to the method developed by Yamakawa et al. (2022) in 2025.

Laboratory-scale shochu production was conducted by Kirishima Shuzo Co., Ltd. using a mash composition consisting of 0.5 kg of koji rice, 2.5 kg of sweetpotatoes, and 2010 ml of water. Distillation was performed under atomospheric pressure using a small glass distiller. In a separate trial conducted by Okuchi Shuzo Co., Ltd., shochu was producted using 1.0 kg of koji rice, 5.0 kg of sweetpotatoes, and 3900 ml of water, and distilled under atmospheric pressure using a small stainless-steel distiller. The alcohol concentration of the secondary mash, the main fermentation stage in shochu production, was measured using the official analytical method prescribed by the National Tax Agency of Japan. In the sensory evaluation, the taste, aroma, and overall flavor balance of the shochu were comprehensively assessed.

Results

Morphological description

The growth habit of Michishizuku was ‘spreading’. Anthocyanin coloration of the nodes and internodes of Michishizuku was classified as ‘absent or very weak’. The leaves of Michishizuku had three very shallow lobes in the leaf blade. The color on both the upper and lower sides of Michishizuku leaves was green. The extent and intensity of anthocyanin coloration on abaxial veins on the lower side of the leaf blade were classified as ‘absent or very small’ and ‘very weak’, respectively. Anthocyanin coloration of the nectary of the leaf blade was absent.

The storage root shape of Michishizuku was elliptic and the flesh color was light beige (Fig. 2). Michishizuku had particolored pink/light beige skin storage roots, namely, the skin color around both ends of the storage roots was pink and the remainder was light beige. The storage root eyes were shallow.

Fig. 2.

Storage roots of Michishizuku.

Agronomic characteristics

ANOVA of total yield, dry matter content, starch content, starch yield, average size of the storage root, and number of storage roots per hill in standard cultivation was performed (Table 1). All the parameters were significantly different among the varieties and in different years. In addition, there were significant year by variety interactions in all the parameters.

Table 1.Analysis of variance of several parameters among four sweetpotato varieties in a four-year trial of standard cultivation

Parameter Sources d.f. Sum of square F-value
Total yield Variety 3 112611.11 43.4121***
Year 3 36082.40 13.9099***
Year × variety 9 37760.74 4.8523***
Error 32 27669.35
Total 47 214123.61
Dry matter content Variety 3 104.28 22.9543***
Year 3 92.86 20.439***
Year × variety 9 30.37 2.2283**
Error 32 48.46
Total 47 275.97
Starch content Variety 3 105.31 72.2015***
Year 3 29.20 20.0217***
Year × variety 9 15.96 3.6469**
Error 32 15.56
Total 47 166.04
Starch yield Variety 3 12587.35 73.5600***
Year 3 3384.04 19.7762***
Year × variety 9 2747.47 5.3520***
Error 32 1825.25
Total 47 20544.10
Average size of the storage root Variety 3 16364.73 10.7764***
Year 3 67010.12 44.1273***
Year × variety 9 11158.28 2.4493*
Error 32 16198.03
Total 47 110731.16
No. of storage roots per hill Variety 3 5.95 10.0142***
Year 3 18.70 31.458***
Year × variety 9 7.07 3.9616**
Error 32 6.34
Total 47 38.06

*, **, *** Significant at the 5%, 1%, and 0.1% levels, respectively.

The total yield of Michishizuku in standard cultivation was 429 kg/a on average for 4 years, which was significantly higher than that of Koganesengan (341 kg/a), higher than that of Shiroyutaka (378 kg/a), and lower than that of Konaishin (468 kg/a) (Table 2). The total yield of Michishizuku in long-term cultivation was 553 kg/a on average for 3 years, which was higher than those of Koganesengan (457 kg/a) and Shiroyutaka (484 kg/a) and lower than that of Konaishin (668 kg/a) (Table 2). The average size of the storage root in standard cultivation was 237 g, which was similar to those of Shiroyutaka (253 g) and Konaishin (255 g), and significantly bigger than that of Koganesengan (209 g). The average size of the storage root in long-term cultivation was 295 g, which was similar to those of Koganesengan (287 g) and Shiroyutaka (333 g), and significantly smaller than that of Konaishin (410 g). The number of storage roots per hill in standard cultivation was 4.9, which was similar to those of Konaishin (5.0) and Koganesengan (4.5), and more than that of Shiroyutaka (4.1). The number of storage roots per hill in long-term cultivation was 6.4, which was similar to those of Koganesengan (5.5) and Konaishin (5.6), and significantly more than that of Shiroyutaka (5.2). The dry matter contents of Michishizuku in standard and long-term cultivations were 40.0 and 41.0%, respectively, which were significantly higher than those of Koganesengan (35.8 and 35.4%), Shiroyutaka (37.3 and 36.3%), and Konaishin (37.4 and 37.5%). Similarly, the starch contents of Michishizuku in standard and long-term cultivations were 28.6 and 30.4%, respectively, which were significantly higher than those of the other varieties. The starch yield of Michishizuku in standard cultivation was 123 kg/a, which was significantly higher than those of Koganesengan (84 kg/a) and Shiroyutaka (99 kg/a) and similar to that of Konaishin (121 kg/a). Similarly, the starch yield of Michishizuku in long-term cultivation was 168 kg/a, which was significantly higher than those of Koganesengan (114 kg/a) and Shiroyutaka (126 kg/a) and similar to that of Konaishin (180 kg/a).

Table 2.Comparative performance of Michishizuku and control varieties

Variety Cropping type Total yieldc (kg/a) Average size of the storage root (g) No. of storage roots per hill Dry matter content (%) Starch content (%) Starch yieldd (kg/a)
Michishizuku standarda 429 ab 237 a 4.9 a 40.0 a 28.6 a 123 a
Koganesengan 341 c 209 b 4.5 ab 35.8 c 24.5 c 84 c
Shiroyutaka 378 bc 253 a 4.1 b 37.3 b 26.2 b 99 b
Konaishin 468 a 255 a 5.0 a 37.4 b 25.8 b 121 a
Michishizuku long-termb 553 ab 295 b 6.4 a 41.0 a 30.4 a 168 a
Koganesengan 457 b 287 b 5.5 ab 35.4 b 25.0 c 114 b
Shiroyutaka 484 b 333 b 5.2 b 36.3 b 26.1 bc 126 b
Konaishin 668 a 410 a 5.6 ab 37.5 b 27.0 b 180 a

Values within a column followed by different letters are significantly different according to Tukey’s test (P = 0.05).

a The planting dates were May 8, 2019, April 23, 2020, May 10, 2021, and May 16, 2022. The harvest dates were October 8, 2019, October 14, 2020, October 19, 2021, and October 5, 2022. Black biodegradable film was mulched. Yield data recorded from a single-row plot 4.2 m long with 35-cm plant spacing and 4 replications. Average of 3 sites each year.

b The planting dates were April 23, 2019, April 17, 2020, and April 19, 2021. The harvest dates were November 5, 2019, November 4, 2020, and November 16, 2021. Transparent plastic film (2019–2020) and black film (2021) were mulched. Yield data recorded from a single-row plot 4.5 m long with 45-cm plant spacing and 4 replications. Average of 2 sites each year.

c Total yield includes storage roots >50 g.

d Starch yield was calculated from the total yield and starch content.

In terms of physiological traits in the storage roots, the deep grooves shown in Koganesengan were rare in Michishizuku. The few skin veins, which appear as blood vessel-like patterns on the surface of storage roots, were slightly observed in Konaishin, but were absent in Michishizuku as in Koganesengan and Shiroyutaka. Michishizuku was slightly more susceptible to storage root crack than Shiroyutaka, and more susceptible than Koganesengan and Konaishin.

An evaluation of the suitability of Michishizuku for shochu brewing revealed that both the alcohol content of the second mash (the main fermentation stage in shochu production) and the yield of alcohol were higher than those of Koganesengan (Table 3). The average sensory evaluation score of shochu made from Michishizuku (Michishizuku shochu) was slightly higher than that of Koganesengan (Koganesengan shochu) and the number of evaluators who had a positive impression was also higher for Michishizuku shochu than for Koganesengan shochu. In addition, Michishizuku shochu exhibited flavor attributes such as sweetness, sweet aroma, raw material-derived notes, and ester-like notes, which are sensory features similar to those observed in Koganesengan shochu. Approximately half of the evaluators in 2020 and about 70% in 2021 rated the flavor profile of Michishizuku shochu as similar to that of Koganesengan shochu, suggesting that Michishizuku shochu exhibits comparable flavor characteristics and quality. Based on these results, Michishizuku is considered a suitable raw material for shochu production.

Table 3.Shochu brewing tests and sensory evaluation of shochu (2019–2021)

Year Variety No. of evaluators Alcohol content of the second mash (%) Yield of alcohol (L/ton for the storage root) Evaluation scorea No. of evaluators with a positive impression Similarity with KS shochub Sensory evaluation commentsc
Kirishima Shuzo
2019 Michishizuku 20 16.9 239 3.4 6 raw material-derived notes, sweet aroma, ester notes, sweetness
Koganesengan 16.0 221 3.2 4 raw material-derived notes, sweet aroma, sweetness
2020 Michishizuku 23 16.7 242 3.7 10 12 sweetness (17), sweet aroma (15), raw material-derived notes (10)
Koganesengan 15.3 220 3.0 6 raw material-derived notes (17), sweetness (17), sweet aroma (15)
2021 Michishizuku 15 16.9 243 3.8 8 10 sweet aroma (13), sweetness (12), raw material-derived notes (9)
Koganesengan 15.1 217 3.2 5 sweet aroma (12), sweetness (12), raw material-derived notes (10)
Okuchi Shuzo
2019 Michishizuku 15.4 201
Koganesengan 14.7 191

Shochu brewing tests and tasting were conducted by Kirishima Shuzo Co., Ltd. (2019–2021) and Okuchi Shuzo Co., Ltd. (2019).

a Evaluation scores were averaged by the assessors using a five-point scale (1–5). The higher the number, the higher the rating.

b Similarity with KS (Koganesengan) shochu indicates the number of evaluators who rated the quality of the shochu (aroma and flavor) as similar to that of Koganesengan shochu.

c The number in parentheses indicates the number of evaluators.

The starch pasting temperature of Michishizuku was the same as that of Koganesengan and slightly lower than those of Shiroyutaka and Konaishin as assessed using a Rapid Visco Analyzer (Table 4). Significant differences in peak viscosity and breakdown were not observed among the four varieties. The setback of starch of Michishizuku was not significantly different from those of the three other varieties. The whiteness of starch of Michishizuku was the same as that of Shiroyutaka, slightly higher than that of Konaishin, and higher than that of Koganesengan.

Table 4.Pasting properties and whiteness of sweetpotato starch (Means of 2019–2022)

Variety Pasting temperature (℃) Peak temperature (℃) Peak viscosity (RVU) Breakdown
(RVU)
Setback
(RVU)
Whitenessa
Michishizuku 74.7 b 82.2 bc 199.7 a 96.2 a 122.4 ab 91.2 a
Koganesengan 74.9 b 81.6 c 193.7 a 87.4 a 118.4 b 88.3 b
Shiroyutaka 76.2 a 83.7 ab 206.9 a 91.1 a 128.3 a 90.6 a
Konaishin 76.7 a 84.3 a 200.5 a 88.1 a 121.0 ab 90.3 ab

Starch samples were prepared from storage roots grown by standard mulching cultivation. The pasting properties of starch were measured using a rapid visco-analyzer RVA-3 (FOSS Japan) at 7% (w/w) starch concentration. Values within a column followed by different letters are significantly different according to Tukey’s test (P = 0.05).

a The whiteness of starch was measured by Powder Whiteness Tester C-130 (Kett).

Disease evaluations

Michishizuku exhibited moderately strong resistance to foot rot (Table 5). In the field infested with foot rot, the incidence of disease was markedly lower than that on Koganesengan, but was not significantly different from that on the moderately strong resistant varieties Konaishin and ‘Benimasari’ (Ishiguro et al. 2004). The yield of the marketable storage roots of Michishizuku was significantly higher than that of Koganesengan, lower than that of Konaishin in 2021, and similar to that of Konaishin in 2022. The ratio of the marketable storage roots of Michishizuku was 51% in 2021 and 83% in 2022, which did not significantly differ from that of Konaishin. Michishizuku exhibited stronger resistance to southern root-knot nematode race SP1, which is the predominant race of M. incognita in Kumamoto prefecture, than Koganesengan, which was rated as susceptible, and similar resistance to Konaishin and Shiroyutaka. Michishizuku exhibited moderately strong resistance to the coffee root-lesion nematode and soil rot. The resistance class of Michishizuku to black rot and fusarium wilt were classified as “medium”.

Table 5.Evaluation of Michishizuku and control varieties for resistance to foot rot disease

Variety 2021 2022
Disease
incidencea (%)
Yield of marketable storage rootsb (kg/a) Ratio of marketable storage rootsc (%) Resistance
evaluationd
Disease
incidencea (%)
Yield of marketable storage rootsb (kg/a) Ratio of marketable storage rootsc (%) Resistance
evaluationd
Michishizuku 33 cd 147 b 51 b moderately strong 29 cde 389 a 83 a moderately strong
Koganesengan 90 a 24 c 16 c weak 83 ab 125 bc 59 b moderately weak
Shiroyutaka 33 cd 150 b 67 ab moderately strong 64 b 126 bc 53 bc moderately weak
Tamaakane 4 d 342 a 92 a (strong) 3 e 431 a 98 a (strong)
Konaishin 28 cd 272 a 68 ab (moderately strong) 10 de 406 a 91 a (moderately strong)
Benimasari 32 cd 97 bc 45 b (moderately strong) 26 cde 96 bc 39 cd (moderately strong)
Ayamurasaki 47 bc 78 bc 58 b (medium) 33 cd 171 b 90 a (medium)
Kokei No.14 75 ab 18 c 12 c (moderately weak) 57 bc 65 c 43 bcd (moderately weak)
Daichinoyume 100 a 10 c 10 c (weak) 100 a 71 c 30 d (weak)

Michishizuku and control varieties were grown in a field infested with foot rot for two years (2021 and 2022), and evaluated for resistance to foot rot. Tamaakane, Konaishin, Benimasari, Ayamurasaki, Kokei No.14, and Daichinoyume were examined as indicator varieties. The level of resistance to foot rot for each indicator variety is indicated in parentheses.

The planting dates were May 6, 2021 and May 1, 2022. The harvest dates were October 13, 2021 and October 17, 2022. Disease incidence was examined on September 2, 2021 and October 7, 2022.

Values within a column followed by different letters are significantly different according to Tukey’s test (P = 0.05).

a Disease incidence = Percentage of plants rotted at the basal part of a stem.

b Marketable storage roots were defined as those that showed no foot rot symptoms or field sprouting.

c Ratio of marketable storage roots = Yield of marketable storage roots / Total yield of storage roots.

d The resistance class to foot rot was evaluated based on the disease incidence, considering the marketable storage root yield and ratio.

Discussion

Michishizuku is a variety developed to replace Koganesengan as a raw material for shochu. It is high yielding, has high starch content, is resistant to foot rot, and is also sufficiently versatile to be used as a starch material.

The total yields of Michishizuku in standard and long-term cultivations were 126 and 121% those of Koganesengan, respectively (Table 2). In comparisons with varieties for starch material, the total yield of Michishizuku in standard cultivation was 113% that of Shiroyutaka and 92% that of Konaishin, while in long-term cultivation, it was 114% that of Shiroyutaka and 83% that of Konaishin. Therefore, the yield of Michishizuku was higher than that of Koganesengan, slightly higher than that of Shiroyutaka, and slightly lower than that of Konaishin. However, since the starch content in the storage root of Michishizuku was 4.1 to 5.4 points higher than that in Koganesengan, 2.4 to 4.3 points higher than that in Shiroyutaka, and 2.8 to 3.4 points higher than that in Konaishin, the starch yield of Michishizuku was 146 to 147% that of Koganesengan, 124 to 133% that of Shiroyutaka, and 93 to 102% that of Konaishin. No significant differences were observed in starch yield between Michishizuku and Konaishin, demonstrating that Michishizuku is a high-yielding variety not only for shochu material, but also for starch material. Since the alcohol present in shochu is made from starch, a high starch content is directly linked not only to the starch yield, which is important as a raw material for starch, but also to the yield of alcohol in shochu brewing, which is also important as a raw material for shochu. Shochu brewing tests confirmed that the alcohol content of the secondary mash of Michishizuku was 0.7 to 1.8 points higher than that of Koganesengan, and the alcohol yield of Michishizuku was 105 to 112% that of Koganesengan (Table 3).

A previous study revealed that the flavors of shochu varied depending on the sweetpotato variety used as the raw material for brewing (Kamiwatari et al. 2006). Koganesengan Shochu exhibits a distinctive flavor profile that is mellow and smooth, free from off-flavors, and most notably characterized by a sweet aroma and sweetness strongly reminiscent of steamed sweetpotatoes, which are considered the defining attributes of its flavor. This type of shochu is highly valued by consumers. Furthermore, Koganesengan is a high-yielding variety with a high alcohol yield and is easy to procure. Consequently, it has been used as the main raw material for shochu production over many years. ‘Joy White’ (Yamakawa et al. 1995), ‘Tokimasari’ (Katayama et al. 2009), ‘Tamaakane’ (Sakai et al. 2011), ‘Satsumamasari’ (Katayama et al. 2013), ‘Koganemasari’ (Sakai et al. 2013), and other varieties have been developed as raw materials for shochu. However, the flavors of shochu made from these varieties differ from that of Koganesengan shochu. Although they have contributed to diversifying the flavor of shochu, they have not become a substitute for Koganesengan. Therefore, alternative varieties to Koganesengan are required not only for cost advantages, such as higher alcohol yields and the easier handling of raw materials, but also to achieve a similar flavor to that of Koganesengan shochu. In a sensory evaluation of shochu, Michishizuku shochu exhibited flavor attributes such as sweetness, sweet aroma, and raw material-derived notes, which were similar to those observed in Koganesengan shochu. Sensory evaluations conducted in 2020 and 2021 further supported this similarity; more than half of the evaluators rated the flavor profile of Michishizuku shochu as similar to that of Koganesengan shochu (Table 3).

Yamamoto et al. (2021) evaluated the brewing characteristics of shochu by analyzing the concentrations of characteristic flavor compounds, including linalool, α-terpineol, citronellol, nerol, geraniol, and β-damascenone, in shochu made from Michishizuku and Koganesengan. Although the concentration of β-damascenone, a compound contributing to a sweet aroma, was slightly higher in Michishizuku shochu, the levels of the other characteristic flavor compounds were nearly the same in both varieties of shochu, indicating that there were no substantial differences in their flavor compound profiles. Taken together, these findings suggest that Michishizuku shochu has a flavor profile that resembles that of Koganesengan shochu, despite exhibiting slightly enhanced sweetness. Therefore, Michishizuku can be regarded as highly suitable for shochu production in terms of its flavor characteristics.

Koganesengan has some disadvantages, the most serious of which is its susceptibility to foot rot. Its resistance class is rated “moderately weak”. The level of resistance of Michishizuku to foot rot was slightly lower than that of Konaishin, but was classified as “moderately strong”, the same class as Konaishin (Table 5). In the resistance test, the disease incidence of Michishizuku was significantly lower than that of Koganesengan and similar to that of the moderately strong resistant varieties Konaishin and Benimasari. In addition, the yield of the apparently healthy storage roots of Koganesengan was 24 kg/a in 2021 and 125 kg/a in 2022, while that of Michishizuku was 147 kg/a in 2021 and 389 kg/a in 2022, which was approximately 3- to 6-fold higher than that of Koganesengan. Control measures for foot rot include practices such as using virus-free seedlings, disinfecting seedlings, and applying chemical treatments. Among these measures, the use of resistant varieties is considered an effective and cost-efficient measure. Michishizuku is expected to contribute to reducing damage caused by foot rot as the first resistant variety capable of replacing Koganesengan.

In addition, while Koganesengan was susceptible to major sweetpotato diseases and pests, Michishizuku was resistant to the southern root-knot nematode, coffee root-lesion nematode, and soil rot, and exhibited moderate resistance to fusarium wilt and black spot disease, suggesting its potential for stable production. Furthermore, the shape of the storage root of Michishizuku is smooth and elliptical without deep grooves, which is superior to that of Koganesengan.

The viscosity characteristics of the starch of Michishizuku were similar to those of Shiroyutaka and Konaishin, and the whiteness of its starch was as high as that of Shiroyutaka (Table 4). Therefore, Michishizuku is also suitable as a raw material for starch. The cultivation of Konaishin has been expanding, with a cultivation area of 1,631 ha in 2022, exceeding that of Shiroyutaka at 1,592 ha. However, Konaishin has the disadvantage of the root stalk connecting the storage root to the stem being very strong, which makes it difficult and time-consuming to separate storage roots from the stem at harvest. The strength of the root stalk of Michishizuku was found to be weaker than that of Konaishin and similar to that of Koganesengan (data not shown); therefore, it may be easier to separate the storage roots of Michishizuku at harvest. These results indicate that it may be preferable to select Michishizuku or Konaishin as the variety for starch material, taking into account factors such as yield, regional adaptability, resistance to diseases and pests, and work efficiency during harvesting.

In southern Kyushu, the damage caused by foot rot has decreased due to efforts to control the disease. One of the management strategies employed involves the use of resistant varieties, which is expected to contribute to reductions in damage caused by foot rot, and the stable production of shochu and starch by expanding Michishizuku instead of Koganesengan in areas where varieties for raw materials for shochu are produced and together with Konaishin in areas where varieties for starch material are produced.

Author Contribution Statement

AK, YK, TS, KS, YK, TS, and YT contributed to the breeding of Michishizuku. AK drafted the manuscript, and all authors approved the final manuscript.

 Acknowledgments

We thank the party of Miyazaki, Kagoshima, Nagasaki, Tokushima prefectures for conducting the performance tests for the recommended varieties, local adaptability tests, and disease resistance tests, and also Kirishima Shuzo Co., Ltd. and Okuchi Shuzo Co., Ltd. for their cooperation in conducting the shochu brewing trials and Mrs. Rieko Gombori, Kumiko Tokudome, Hiroko Ikeda, and the members of Kyushu Operation Unit 3 of the Technical Support Center of the Kyushu-Okinawa Region and Miyakonojo Research Station (KARC/NARO) for their technical assistance. This work was supported by the research program on the development of innovative technology grants (JPJ007097) from the Project of the Bio-oriented Technology Research Advancement Institution (BRAIN).

Literature Cited
 
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