Takashi Sayama1) 2),
Masayasu Saruta2) 4),
1) Institute of Crop Science, National Agriculture and Food Research Organization (NARO)
2) Western Region Agricultural Research Center, NARO
3) Kazusa DNA Research Institute
4) Present address: Agriculture, Forestry and Fisheries Research Council, Ministry of Agriculture, Forestry and Fisheries
5) Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University
Ln gene, Glycine max (L.) Merrill, pleiotropic effect, leaflet shape, seed number per pod
The final version of this article with its full bibliographical information is available. To access the article, click here (Vol. 67 (2017), No. 4).
Most soybean cultivars possess broad leaflets; however, a recessive allele on the Ln locus is known to cause the alteration of broad to narrow leaflets. The recessive allele ln has also been considered to increase the number of seeds per pod (NSP) and has the potential to improve yield. Recently, Gm-JAG1 (Glyma20g25000), a gene controlling Ln, has been shown to complement leaf shape and silique length in Arabidopsis mutants. However, whether Gm-JAG1 is responsible for those traits in soybean is not yet known. In this study, we investigated the pleiotropic effect of soybean Ln gene on leaflet shape and NSP by using two independent soybean Gm-jag1 mutants and four ln near isogenic lines (NILs). The leaflet shape was evaluated using a leaf image analysis software, SmartLeaf, which was customized from SmartGrain. The leaflets of both the Gm-jag1 mutants were longer and narrower than those of the wild-type plants. Interestingly, the image analysis results clarified that the perimeter of the mutant leaflets did not change, although their leaflet area decreased. Furthermore, one mutant line with narrow leaflets showed significantly higher NSP than that in the wild (or Ln) genotype, indicating that soybean Ln gene pleiotropically controls leaflet shape and NSP.
Edited and published by : Japanese Society of Breeding Produced and listed by : Nakanishi Printing Co., Ltd.