Culture in vitro of Excised Ovaries in Brassica campestris L. : II. Development of Excised Ovaries In Various Carbon Sources

Abstract

Studies on development in vitro of excised ovaries in the sibmate of diploid Brassica campestris L. ssp. chinensis (L.) Makino cv. Seppaku-taina and in the sibmate of autotetraploid B1'assica campestris L. ssp. pekihelesis (Lour.) Olsson cv. Nozaki-hakusai were carried out. Development in vitro of excised ovaries at four days after pollination was studied in various carbon sources and various sucrose concentrations. Results obtained were as fellows. (1) Seed set in the capsules was observed in culture media with sucrose, glucose, remnose and fructose in each sibmate of diploid Seppaku-taina and autotetraploid Nozaki-hakusai. (2) Neither growth of the capsules nor seed set in the capsules were observed in the medium with mannose in both sibmates. (3) In diploid Seppaku-taina, seed set in the capsules was better in the medium with sucrose or giucose than with fructose or raffilnose. (4) In autotetraploid Nozaki-hakusai, seed set in the capsules was better in the medium with sucrose, glucose or raffinose than with fructose. (5) In the experiment of various sucrose concentrations on development ile vitro of excised ovaries, the higher Sucrose concentration in the medium, the better seed set in the capsules was observed in diploid Seppaku-taina. Seed set in the capsules was best in the medium with 210g/l of sucrose. Percentage of germinated seeds was, however, somewhat less than that from the seed obtained in the medium with lower sucrose concentration. (6) In autotetraploid Nozaki-hakusai, seed set in the capsules was good from 30g/l to 70g/l of sucrose in the medium. (7) Seed set in the capsules in development ile vitro of excised ovaries was different between diploid Seppaku-taina and autotetraploid Nozaki-hakusai.