Abstract
Protoplasts from callus tissues of rlce, Oryza sativa L., and soybean, Glycilee max (L.) Merrill., were cultured in liquid media. Cell division occurred in protoplasts of both plants and resulted in the formation of calluses or cell colonies after one or two months. Protoplasts of both plants were Lused by using polyethyleneg'lycol (PEG)-high pH-high Ca++ technique. The percentages of hybrld protoplasts obtalned by fusron of rice and soybean were approximately 1-3 percent. The hybrid protoplasts gave rise to cell clusters after two weeks of successive culture. The cell clusters of hybrid cell lines could be distinguished by features of nucleus and cytoplasm frorn those originated from non-hybrid cells of rice and soybean.
