Abstract
Since the principal injury of A1 on crop plants is an inhibition of root cell division, most of the methods for screening for A1 tolerance are based on measuring inhibition of root growth. This approach has some inconvenient procedures as a screening method for A1 tolerance, for example, it requires much time for the measurements and the complexity of maintaining the pH of the nutrient solution. It is known that crop species or varieties tolerant to A1 accumulate less A1 in their roots than susceptible ones (VOSE et al. 1962). It was recently reported that hematoxylin staining of A1 absorbed in wheat roots was of use for detecting A1 tolerance (POLLE et al. 1978). Therefore, ¥ve have conducted experiments to evaluate the hematoxylin staining method from the viewpoint of practical wheat breeding.
