Abstract
Protoplasts isolated from nucellar callus of 'Trovita' orange (Citrus sinensis Osbeck) wvere cultured in a MURASHIGE and TUCKER'S medium containing 0.15 M sucrose, 0.45 M glucose, and 0.6% agar, but lacking phytohormones. Colonies with 26.3% plating efficiency were observed after 6 weeks culture. When the protoplasts were cultured in the presence of exogenous phytohormones, reduction in colony formation was observed. Some colonies developed to green embryoids, which eventually formed plants.
