Induction of Adventitious Buds and Globular Embryoids on Seedlings of Trifoliate Orange (Poncirus trifoliata)

Abstract

The adventitious buds and globular embryoids were obtained from a nucellar seedling of a trifoliate orange by the use of two kinds of plant hormones, i. e., α-naphthaleneacetic acid (NAA) and 6-benzyl aminopurine (BA). The highest frequency of induction of the adventitious buds was obtained, when 1.0 mg/l NAA and 5.0 mg/l BA were supplemented to the culture medium. Adventitious root formation was promoted by the supplement of 2.0mg/l NAA to the culture medium. A micropropagation scheme for a trifoliate orange was put forward.