Japanese Journal of Breeding
Online ISSN : 2185-291X
Print ISSN : 0536-3683
ISSN-L : 0536-3683
Production of Blue Pigment in Leaf-derived Callus of Lavender (Lavandula vera DC)
Masato TsuroMasayoshi Inoue
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Keywords: suspension culture
JOURNAL FREE ACCESS

1996 Volume 46 Issue 4 Pages 361-366

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Abstract
Leaf-derived calli of lavender (Lavandula vera DC) were obtained on the Murashige and Skoog solid medium supplemented wit, h 2, 4-dichlorophenoxyacetic acid (2, 4-D) and kinetin, or, 3-indole butylic acid (IBA) and 6-benzy-ladenine (BA), at 25°C under dark condition. Callus formation rate on the medium with 2, 4-D and kinetin was higher than that with IBA and BA. Some of calli obtained on the medium with 2, 4-D and kinetin, produced blue pigment. On the other hand, in the combination of 1×10-6 M 2, 4-D and 1×10-6 M kinetin, calli grew vigorously, but they did not produce blue pigment. In the medium with 1×10-5 M 2, 4-D and 2×10-5 M kinetin, most of calli produced blue pigment. These calli continued to produce blue pigment after subculture to the medium with same components. However, after transfer to the medium with 1×10-6 M 2, 4-D and 1×10-6 M kinetin, they did not produce blue pigment.
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