Abstract
Severe sterility barriers of interspecific unilateral and cross incompatibility exist between Lycopersicon esculentum and "peruvianum -complex". In particular, the strictest barriers hamper the embryo development of the hybrid between L. esculentum and L. peruvianum var. humifusum. This study was carried out in order to produce the interspecific hybrids and their backcross progenies using the ovule selection method (Imanishi et al. 1993). A total of 23 hybrid plants (F1) were obtained from 67 fruits. Hybrid nature of these plants were confirmed by the morphological and physiological resemblance of their traits to the pollen parent, L. peruvianum var. humifusuin LA 2153. The BIFI plants obtained were 9 plants from 61 fruits. Three of them were inferred to be self-compatible plants from their fruit set behaviour. Many plants of the B2F1 were produced, of which 77 % were self-compatible. PCR analysis of B2F2 plants was performed in order to confirm the introgression of the acid-invertase gene of the L. peruvianum var. humifusum which is associated with sucrose accumulation in a fruit.
