1999 Volume 49 Issue 2 Pages 89-95
In order to apply isozymes as genetic markers tn sugarcane breeding, the polymorphism of 24 Japanese varieties, including breeding nraterials, was analyzed using 12 enzyme systems. Of the enzymes examined, the activity of Alcohol dehydrogenase (ADH), Esterase (EST), Glutanrate oxaloacetate transaminase (GOT), Isocitric dehydrogenase (IDH) and Peroxidase (POX) was clearly revealed and polymorphism was detected among the varieties. The 24 varieties could be identified by these five enzyme systems. In the selfed progenies of NiF8, Ni9 and RK86-129, Iarge numbers of bands in EST and POX were segregated. Segregation patterns in some of the isozymes in EST and POX showed a monogenic segregation ratio of 3 : 1. However, others deviated from the monogenic segregation ratio. In a parentage test using isozymes, the hybridity of alnrost all the seedlings could be identified in 10 cross combinations. These results indicate that the isozyme technique is a useful tool for variety identification and progeny hybridity in sugarcane breeding.
