2017 Volume 8 Issue 2 Pages 127-131
【Objective】We compared the clearance characteristics of creatinine, interleukin (IL)-8 and IL-6 during continuous hemofiltration (CHF) using three different materials for the hemofilter and different filtration flow rates in vitro. 【Methods】Phosphate buffer solutions containing creatinine, IL-8 and IL-6 were produced as test solutions. A CHF circuit was prepared using polysulfone (PS), polymethyl methacrylate (PMMA) and acrylonitrile-co-methallyl sulfonate surface-treated (AN69ST) hemofilters. The test solution flow rate was set to 150mL/min. The filtration flow rates were set to 600, 1,200 and 2,100mL/hr. Sampling of the test solution was performed inlet and outlet each hemofilter. Samples were collected 6 times over a period of 9 min after initiation of the CHF procedure. The concentration of creatinine and the concentrations of IL-8 and IL-6 were determined by an enzymatic method and ELISA, respectively. 【Results】There were no significant differences in creatinine clearance with the three hemofilter materials. Regardless of the hemofiltration material, the clearance of creatinine increased with an increase in filtration rate. However, clearances of IL-8 and IL-6 with PMMA and AN69ST hemofilters were significantly higher than that with the PS hemofilter, and the clearance of these cytokines were not associated with filtration flow rate. 【Conclusion】Clearance of large molecular weight substances such as cytokines in CHF using an adsorbing type of hemofilter is superior to that in CHF using a filtration type of hemofilter. The results suggest that selection of an adsorbing hemofilter is important for treatment of sepsis when performing CHF for removal of cytokines.
