Abstract
A new method for quantitative determination of polyamines in tissues is developed in comparison with the alkaline butanol method which has been widely employed for extraction of polyamines from tissues.
The procedures consist of isolation of polyamines with a small column of Dowex-50 and their separation on paper electrophoresis followed by colorimetric determination after ninhydrin staining.
Recovery by the present method is constantly higher than 90%, with an average value of 95%, whereas the butanol extraction results in variable recoveries ranging from 70 to 90%. Our method is also superior to the butanol method from the standpoint of purity of the isolated polyamines and simplicity of the procedures.
The column method was applied to quantitative estimation of polyamines in various tissues of rat.
