抄録
Zymograms of urinary isoamylase were obtained by means of agar electrophoresis on patients with total pancreatectomy, acute pancreatitis, acute parotitis, gallstone and pancreatic cancer, and were compared with those of pure pancreatic juice, pancreatic extract and saliva. Amylase activity in each piece of agar, which was cut into 25 pieces after electrophoresis, was determined by the iodometric method.
It was suggested that the main fraction of pancreatic and salivary isoamylases of urine could be separated. In addition, the increase in activity of pancreatic amylase was found in patients with acute pancreatitis and the decrease in activity in patients with pancreatic cancer with jaundice after pancreozymin-secretin sequence of injection as compared with that of control.
The increase in the urinary pancre atic amylase activity was found in patients with both cholesterol gallstone and pigment gallstone, and there was long-standing high pancreatic amylase activity in patients with cholesterol gallstone after causal operation. On the other hand, the reduction of the activity to normal was found in patients with pigment gallstone.
There was an increa se in urinary pancreatic amylase activity in a hamster with pure cholesterol gallstone experimentally produced by a 20% butter diet or a fat-free diet for 4 weeks. There was a decrease in pancreatic amylase activity in the hamster, which showed histologically some pancreatic fibrosis produced by a 20% butter diet for 8 weeks, as compared with control.
These data show one of the evidences that nutritional factors are of etiologic significance in pathogenesis of pancreatitis combined with gallstones.
