抄録
A simplified colorimetric assay for blood levels of pyruvate kinase activity has been developed for the clinical use. After incubation of 0.1ml serum (or plasma) with substrates at pH 7.4 for 10min, pyruvate formed was detected as hydrazone formation by absorbance change at 510nm.Intraassay variance was 1.5% and the activity was stable for week on storage at-20°.
In 36 cases with acute transmural myocardial infarction, plasma PK levels were all elevated from 3 hours to 4-5 days after the onset of the symptom. The mean peak value was 1761U/l, which was 5 fold over normal.Plasma levels of PK were not elevated in sublects with angina pectoris, acute hepatitis or hypothyroidism and seemed to be more specific to myocardial infarction compared with CPK, GOT and LDH.
These results indicate that the present assay kit for blood level of pyruvate kinase is clinlcally useful for the diagnosis and follow up of myocardial infarction and other muscle diseases.
