Abstract
Three couples of biological guaiacol estrogen isomers (2;3, 5;6, and8;9) and two couples of synthetic ones (11;12, and 14;15) were separated satisfactorily by reversed phase high performance liquid chromatography.
The devised technique involves the elution with a mixture of acetonitrile and water (45: 55) on a column of ODS-SIL monitoring the absorbance at 280nm.
Calibration curves between the amount (μg) of steroid inlected and the peak height (cm) on chrmatogram were linear.The similar linear relationships by internal standard method were obtained for the ratio of guaiacols to estradiol added as an internal standard.
The results for the quantifi Cation of radioactive guaiacols obtained by the incubation of catechol estrogen with rat liver Catechol O-methyltransferase in the presence of [3H-CH3] - S-adenosyl-L-methionine were in good agreement with the results by other two procedures, reversed isotope dilution and radio thin-layer chromato-scanner methods.
