Kinetic Studies on Determination of Serum Cholineesterase Activity Using Choline Oxidase

Abstract

A kinetic studies on enzymic determinationfor serum cholinesterase activity using cholineoxidase (EC 1.1.3.17) is described.
Preparation of purified enzyme of Cholinesterase is capable to determine serum cholinesterase activity enzymically.
The reacted mechanism of choline oxidase for choline has revealed more complicated steps, because the degradation of choline was proceeded on two ways, lst were liberation step of betain aldehyde., 2nd were formation step of BA´-El complex.
The optimum condition for determination of cholinesterase activity were introduced on the basis of above results.