臨床化学
Online ISSN : 2187-4077
Print ISSN : 0370-5633
ISSN-L : 0370-5633
Choline Oxidaseを用いる血清Cholinesterase測定法のKinetic面からの検討
勇 二三雄田中 秀武小川 善資丸井 洋二有末 一隆甲田 一馬林 長蔵
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1981 年 10 巻 2 号 p. 160-168

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抄録
A kinetic studies on enzymic determinationfor serum cholinesterase activity using cholineoxidase (EC 1.1.3.17) is described.
Preparation of purified enzyme of Cholinesterase is capable to determine serum cholinesterase activity enzymically.
The reacted mechanism of choline oxidase for choline has revealed more complicated steps, because the degradation of choline was proceeded on two ways, lst were liberation step of betain aldehyde., 2nd were formation step of BA´-El complex.
The optimum condition for determination of cholinesterase activity were introduced on the basis of above results.
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