Abstract
Enzymatic determination of urinary estriol in pregnancy using thin-layer chromatographic separation has been described. The enzymatic assay of estriol is performed with peroxidase as a catalyzer, hydrogen peroxide as a hydrogen acceptor and estriol as a substrate of hydrogen donor where hydrogen peroxide is consumed depending on the concentration of estriol, and remains of hydrogen peroxide is determined colorimetrically using 4-aminoantipyrine, phenol, and peroxidase. Urinary estriol purified and separated by thinlayer chromatography is determined enzymatically. The correlation obtained by other colorimetric method and this enzymatic method is fairly good.
