Abstract
we established a new procedure for the separation and quantitation of amino acids in human plasma and cerebrospinal fluid using high performance liquid chromatography.
The devised technique involves the step gradient elution with 0.2N citrate buffer (pH3-22, 4. 25) and 0.3 M borate buffer (pH 8.0, 9.9) on a column of 2, 4-divinyl benzene polymer sulfonated cation exchange resin and a high sensitive fluorescence reaction for amino acids by o-phthalaldehyde. The method described here is simple, time-saving, accurate and reproducible compared with the previous method.
For the clinical purposes, plasma specimenes of 75 adult, 58 children and cerebrospinal fluid specimenes of 18 children were subjected to the analysis.
