Abstract
Asimple and rapid high performance liquid chromatographic method for analysis of fluoresclmine-llbeled norepinephrine and dopamine in 2ml of human urine was devebped.About 10 urine samples could be analyzed in 8hours.Coefficient of vlriation for norepinephrine and doplmine for individual urine slmples were 6.5% (52.0±3.0 pmol/2ml, n=7) and 3.8% (1.97±0.08nmol/2ml, n=7), respectively, and those for the another urine were 2.9% (263±8.3 pmol/2ml, n=7) and 4.1% (2.55±0.10 nmol, /2mol n=7), respectively.There was no difference in overnight urinlry excretion rate of norepinephrine and dopamine between the normal and hypertensive groups.After 2 hr of upright posture, the excretion rate of norepinephrine in the patients with essential hypertension was significantly higher (P‹0.05) than that of the normal sublects.After a subsequent 2 hr recumbency, the norepinephrine excretion rate was also higher (P‹0.05) in the hypertensive patients. There were no significant differences in excretion rltes of dopamine between the two groups during any period.
