Abstract
A rapid, selective and sensitive method for the simultaneous determination of chlorpromazine (CPZ) and levomepromazine (LPZ) in human plasma has been developed using high-performance liquid chromatograph with electrochemical detector.
The unchanged drugs and internal standard extracted from plasma were separated by a reversed-phase high-performance liquid chromatography and the separation was completed within 10 min. The influences of acetonitrile concentration and of the pH of the mobile phase were investigated. The minimum detectable amounts were 100pg for CPZ and LPZ, respectively. In comparison with other three detection systems this was found to be the most sensitive method.
This method was applied to the simultaneous determination of CPZ and LPZ in human plasma.
