In order to identify a true antigen responsible for the induction of the asthmatic attack, antigen provocation tests were performed, and the correlation between the pulmonary functions and the changes in plasma lysosomal enzyme activities was obtained.
When DSCG was administered prior to the provocation, release of the lysosomal enzyme activities was significantly prevented, and the asthmatic attack was suppressed or a slight, even if induced.
The lysosomal enzyme activities were lower in the adrenal steroid hormone-dependent patients. Measurement of the plasma lysosomal enzyme activities may give pro missing methods in evaluating a true antigen in place of the pulmonary function tests, and in addition, dependency on the steroidal hormone may be judged by the present biochemical method.
When the antigen, an extract of mites, induced a positive skin test but negative provocation test, lysosomal enzymatic activities did not change significantly in a preliminary test, and the result was excluded from the present report.

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A rapid and sensitive method for the simultaneous gas-chromatographic determination of anticonvulsants (carbamazepin, phenobarbital, primidone and phenytoin) in serum or dried blood spot on filter paper without derivatization is described. The chloroform extract from serum sample is directly injected into a gas-chromatograph equipped with a nitrogen-selective detector, which containes a siliconized glass column, packed with a mixture of 0.25% neopentyl glycolsuccinate polyester-0.025% H₃PO₄(120cm×3mm I.D.) or a ultrabond 20M (90cm×2mm I.D.). The column temperature and flow rate of carrier gas are 230° and 30ml/min, respectively.
The dried blood spot sample is extracted with a mixture of chloroform-methanol-acetic acid (50: 1: 0.01, by volume). After evaporation of the solvent, the residue is dissolved in chloroform and assayed by the above gas-chromatograph. The recoveries from serum and dried blood spot samples are 87.3-103.3% (n=5) and 86.9-92.4% (n=5), respectively. The determination limit of this method is ing to 5ng Comparison between the results obtained from serum and dried blood spot samples gave a correlation coefficients of 0.97 for phenobarbital, 0.95 for primidone, and 0.88 for phenytoin, respectively. The method proposed here is useful for a routine monitoring for the concentration of drug in blood of patients.

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The lipoperoxidation in plasma was the most accelerated by the addition of 8-hydroxyquinoline (8HQ)-Fe (III) chelate among all 8HQ-metal chelate systems examined. The same tendency was also observed for 5-chloro-7-iodo-8HQ (C)-Fe (III) chelate system. The activity of 8HQ-Fe (III) system in lipoperoxidation was the highest at [8HQ]:[Fe (III)]=1: 1. In quinoline derivatives, the ligands which have 8-hydroxyl group and high solubility in lipid were necessary for lipoperoxidation in plasma by Fe (III) chelate systems.
From above facts, both of the denaturation of nervous systems in SMON patients ad ministered with C and the antibiotic effects of 8HQ may be considered as the results of lipoperoxidation by C-Fe (III) chelate and 8HQ-Fe (III) chelate, respectively.

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A rapid, selective and sensitive method for the simultaneous determination of chlorpromazine (CPZ) and levomepromazine (LPZ) in human plasma has been developed using high-performance liquid chromatograph with electrochemical detector.
The unchanged drugs and internal standard extracted from plasma were separated by a reversed-phase high-performance liquid chromatography and the separation was completed within 10 min. The influences of acetonitrile concentration and of the pH of the mobile phase were investigated. The minimum detectable amounts were 100pg for CPZ and LPZ, respectively. In comparison with other three detection systems this was found to be the most sensitive method.
This method was applied to the simultaneous determination of CPZ and LPZ in human plasma.

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Apo C II and C III of serum VLDL were determined in various types of hyperlipidemias by isoelectric focusing of polyacrylamide gel plate with a pH gradient 4.0-6.0. VLDL from hyperlipidemias of type lib, III, IV and hypo α-lipoproteinemia had high C II/C III ratio. Percentage of apo C III-O tended to increase in type III and IV, and decreased in type lla and hypo α-lipoproteinemia. Therefore, apo C ll/C III-O was not changed in type III, IV and V and increased type II a and hypo α-lipoproteinemia. Apo C III-1 did not change in every type of hyperlipidemias when compared with normolipidemic control but apo CIII-2 decreasedin type II b and IV. Decrease of apo C III-2 and increase of apo C III-O in hypertriglyceridemic patients suggests the relative impairment of sialylation of apo C III proteins.E/C ratio was low in type II b, IV and hypo α-lipoproteinemia.
There were no clear relationship between C II/C III or C II/C III-O ratio and serum triglyceride or pre β-lipoprotein concentration. It is concluded that apo C composition of VLDL is not always related to the development of hypertriglyceridemia.

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An assay method for neuraminidase in human lymphocyte has been developed. The method was based on the determination of enzymatically liberated 4-methylumbellife tone (4-MU) by a fluorometric procedure.
Homogenates of lymphocyte cells cleaved the 4-MU from the 4-methylumbelliferyl a-kc to side of N-acetylneuraminic acid. Maximum activity occurred at pH 4.5 in sodium acetate buffer. The Km value was 2.35×10^-4M. The hydrolysis proceeded linearly with the incubationtime up to 150 min and with enzyme protein concentration up to 0.2mg. The enzyme was inhibited by choric acid, sodium laurylsulfate and also by isotonic concentrations of LiCl, KCI, NaCl, and CaCl₂.
The normal values of enzyme activity was somewhat lower in females (2.38±0.90 4-MU /mg protein/h) than in males (3.03±1.30). In contrast, sialic acid concentration of the same lymphocyte was higher in females (87.8±31.sing/g protein) than in males (58.5±32.5).

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