A Simple Radioimmunoassay for Estrone Sulfate in Unextracted Plasma without Deconjugation 1)

Abstract

A new didect radioimmunoassay for the determination of estrone sulfate in unextracted plasma without prior deconjugation has been developed. The procedure involves the use of sodium salicylate and methanol as blocking agents to inhibit the binding of estrone sulfate to plasma proteins and of highly specific anti-estrone sulfate antiserum immobilized on p-arylamine glass beads. The proposed method eliminates extraction of estrone sulfate from plasma, chromatographic separation and prior solvolysis and hence, is much more reliable and favorable than known methods for the routine assay.