Abstract
A new method was described for the separation of lipases by electrophoresis utilizing a cellulose acetate membrane.
Triolein or partially purified-endogenous fat from obese mice was used as a substrate of lipases and liberated fatty acids from substrate hydrolysed by enzymes on the membrane were coupled with copper ion in a solution of copper-triethanolamine. Coupled-copper ion was stained by diphenylcarbazide.
Four bands were found in the zymograms of lipases in the aqueous extracts from different tissues in mice, which were designated ae Lpl, Lp2, Lp3 and Lp4, respectively.
Furthermore, since fractionated lipoprotein lipase from postheparin plasma by heparin affinnity column chromatography was also observed by zymography, it was known that a new method wight be useful for investigation of lipoprotein lipase.
