抄録
Proteins were separated by high performance liquid chromatography (HPLC) using anion-exchange and gel-permeation type of resin. Human plasma was separated into 4 peaks by gel-permeation HPLC. On the anion-exchange type of HPLC, human plasma was separated into 15-16 peaks in only less than 30 min and elution profile was resemble to the pattern of electrophoresis. Ovalbumin which was eluted in single peak on gel-permeation HPLC, was separated into 5-6 peaks by anion-exchange chromatography. On the ionexchange HPLC of rat urine, esterase activity was detected in 5 peaks and peptidase acti -vity was detected in 4 peaks without enzymatic activity being interfered. These results suggested that this new HPLC was an excellent technique for the clinical chemistry of protein.
